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Test Code 8BM19 Flow Cytometry - BCP-ALL MRD panel post anti-CD19 immunotherapy

Important Note

Please provide relevant treatment history including monoclonal antibody treatment (e.g. anti-CD19, anti-CD20 and anti-CD22 treatment, etc.) on test request form.

 

Testing is performed on fresh sample and samples should be dispatched to reach the laboratory before 2pm Monday to Friday for processing. As such, do not dispatch samples on eve of Public Holiday, Saturday and Sunday.

 

**Refer to FCM Flow Chart for ordering guide**

 

Specimen Requirements

Submit one of the following:

 

Fresh specimen required

 

(a) Specimen Type: Bone marrow (preferable)

      Container/Tube: Lavender Top (EDTA), Green Top (Sodium Heparin)

      Volume: 5mL

 

(b) Specimen Type: Peripheral blood

      Container/Tube: Lavender Top (EDTA), Green Top (Sodium Heparin)

      Volume: 10mL

      Collection Instructions: Collect 20mL blood if WBC < 5 x 109/L.

 

Additional Collection Instructions: All specimens should be shipped to the lab in ambient temperature (18-25oC) within 24-48 hours of collection. Specimen received after 48 hours of collection will nonetheless be processed but results may be compromised. For overseas specimens, avoid shipping on Friday or eve of public holidays.

 

Reference Interval

See laboratory report

Days and Times Performed

Monday through Friday. 20 working days (STAT 5 days)

Methodology

Flow Cytometry

Service Code

12666

Update

Last updated 22/10/2024

Added new test info

 

Unacceptable Condition

 

Specimen Type
Bone Marrow/Peripheral Blood Frozen                                         

 

Specimen Stability

 ** Preferred temperatures for storage prior to and during shipping to NUH **

 

Fresh specimen required

Ambient (Preferred) 24 hours
Refrigerate 48 hours                   

 

Testing Laboratory

National University Hospital
Flow Cytometry, 67724016

Useful For

This panel is used for follow-up examination of B-ALL post anti-CD19 immunotherapy or CAR-T treatment. The previously detected characteristic aberrant expressions are used for MRD monitoring.

Test Info

Pre-arrange with Haematology before ordering the test.

 

This laboratory test has undergone in-house evaluation with appropriate quality assurance and control measures in line with laboratory best practice and accreditation requirements.

 

Reagent(s) used in this test is/are ordered through HSA’s Special Access Route, which provides interim access without full registration.

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