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Test Code 8CSBL Flow Cytometry - Blast Lineage Panel (NO add-ons)

Important Note

Refer to FCM Flow Chart for ordering guide

This panel is used to determine blast lineage, BUT NOT leukaemia subtype.

Please order Flow Cytometry - Blast Lineage Panel and Evaluation As Required for leukaemia subtype, or Flow Cytometry - Blast Lineage Panel, Lymphoma Screening Panel and Evaluation As Required for subtype of leukaemia or lymphoma.

Laboratory Code

LAB5233

Specimen Requirements

Fresh specimen required. Submit one of the following:

(a) Specimen Type: Bone Marrow

      Container/Tube: Lavender Top (EDTA), Green Top (Sodium Heparin)

      Preferred Volume: 2.0 mL

 

(b) Specimen Type: Peripheral Blood

      Container/Tube: Lavender Top (EDTA), Green Top (Sodium Heparin)

      Preferred Volume: 3.0 mL

      Collection Instructions: Collect 5.0 - 6.0 mL blood if WBC < 5 x 109/L.

     

(c) Specimen Type: Lymph Node / Solid Tumour

      Container/Tube: Sterile container with 5.0 mL RPMI 1640 media

      Preferred Volume: 5 mm(3) or larger biopsy

      Collection Instructions: Place tissue directly into 5.0 mL RPMI 1640 media after biopsy.

      Do not leave tissue to air-dry or fix tissue in formalin.

      (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

     

(d) Specimen Type: Body Fluid

      Container/Tube: Sterile tube with 0.6 mL transfix media

      Preferred Volume: 3.0 - 6.0 mL

      (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

 

(e) Specimen Type: "Small cell number" samples, e.g. CSF, vitreous biopsies

      Container/Tube: Sterile tube with 0.2 mL transfix media

      Preferred Volume: 1.0 - 2.0 mL

      (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

 

(f) Specimen Type: Fine Needle Aspirate

     Container/Tube: Sterile container with 5.0 mL RPMI 1640 media

     Collection Instructions: Place fine needle aspirate directly into 5.0 mL RPMI 1640 media after collection.

     Do not place in formalin or in a sterile container without any tissue culture medium.

      (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

 

Additional Collection Instructions: All specimens should be shipped to the lab in ambient temperature (18 - 25oC) within 24 - 48 hrs of collection. Specimen received after 48 hrs of collection will nonetheless be processed but results may be compromised. For overseas specimens, avoid shipping on Friday or eve of public holidays.

Specimen Stability

Fresh specimen required

Ambient 24 hours (preferred)
Refrigerate 48 hours   

Methodology

Flow Cytometry

Days and Times Performed

Monday through Friday. TAT 14 - 16 days (STAT 7 - 9 days)

Reference Interval

See laboratory report

Testing Laboratory

National University Hospital
Flow Cytometry, 67724016

Useful For

This panel is used to obtain a simple CD34+ blast count and determine blast lineage, BUT NOT leukaemia subtype.

Please order Flow Cytometry - Blast Lineage Panel and Evaluation As Required for leukaemia subtype, or Flow Cytometry - Blast Lineage Panel, Lymphoma Screening Panel and Evaluation As Required for subtype of leukaemia or lymphoma.

Service Code

12650

Update

Last updated 22/01/2025

Test Info

Pre-arrange with Haematology before ordering the test.

 

This laboratory test has undergone in-house evaluation with appropriate quality assurance and control measures in line with laboratory best practice and accreditation requirements.

 

Reagent(s) used in this test is/are ordered through HSA’s Special Access Route, which provides interim access without full registration.

Unacceptable Condition

Specimen Type
Bone Marrow/Peripheral Blood/Body Fluid Frozen
Lymph Node/Solid Tumour/Other Tissues Frozen or air-dry or formalin fixed tissues

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