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Test Code LYMS Flow Cytometry - Lymphocyte Subsets

Important Note

Specimens are acceptable from Monday to Thursday.

Specimen must be RECEIVED by Laboratory before 11.00 AM on Tuesdays and Thursdays.

Do not send specimens on Fridays, Saturdays, Sundays, Public Holidays and after 11.00 AM on Tuesdays and Thursdays.

Laboratory Code

LAB3041534

Specimen Requirements

Specimen Type: Whole Blood
Container/Tube: Lavender Top (EDTA)
Preferred Volume: 3.0 mL
Minimum Volume: 1.0 mL whole blood

Specimen Stability

Specimen Type Whole Blood
Ambient 48 hours
Refrigerate Not available
Frozen Not available

Panel Information

Panel includes the following tests:

  • Lymphocytes, Abs (cells/uL)
  • CD3, T Lymphoytes (%)
  • CD3, T Lymphoytes  Abs (cells/uL)
  • CD4 T Helper Cells (%)
  • CD4 T Helper Cells Abs (cells/uL)
  • CD8 T Suppressor Cells (%)
  • CD8 T Suppressor Cells Abs (cells/uL)
  • CD19, B Cells (%)
  • CD19, B Cells Abs (cells/uL)
  • NK Cells (%)
  • NK Cells Abs (cells/uL)
  • CD4/CD8 Ratio  

Days and Times Performed

Tuesday and Thursday, TAT 7 - 9 working days.

Specimen Condition

Haemolysis OK
Icterus OK
Lipaemia OK
Others: Clotted or insufficient specimen Reject

Reference Interval

  Lymphocyte Absolute Count CD3 T Lymphocytes CD3 T Lymphocytes, Abs CD4 T Helper Cells CD4 T Helper Cells, Abs CD8 T Suppressor Cells CD8 T Suppressor Cells, Abs CD19 B Cells CD19 B Cells, Abs NK Cells NK Cells, Abs CD4/CD8 Ratio
Unit cells/uL % cells/uL % cells/uL % cells/uL % cells/uL % cells/uL Nil
At Birth Male Lower Limit 2000 53 1200 36 800 13.5 400 12 300 16 400 1.9
Upper Limit 3900 74 2000 48 1900 19.5 800 22 600 27 1100 2.5
Female Lower Limit 1400 65 900 44 1000 18.5 400 12 200 9 100 1.7
Upper Limit 2900 76 2100 53 1900 24.5 1000 20 400 16 400 3
2 days - 11 months old Male Lower Limit 2800 56 1800 33 900 16 700 23 700 8 300 1.6
Upper Limit 5700 67 3300 39 2300 24 1500 30 1700 16 800 2.2
Female Lower Limit 4300 60 2800 37 1700 16 800 21 1000 7 300 1.9
Upper Limit 5700 71 3500 48 2400 21 1100 33 1700 14 800 2.9
1 - 6 years old Male Lower Limit 2500 56 1500 29 1000 19 700 18.5 500 9 300 1.1
Upper Limit 5100 68 2900 40 2100 25 1100 28 1200 19 600 2
Female Lower Limit 3100 62 1900 30 1000 20 700 19 600 7 300 1.2
Upper Limit 4700 70 2900 40 1800 27 1200 29 1400 16 600 2
7 - 18 years old Male Lower Limit 1500 65 1100 27 600 23 500 15 200 11 300 1
Upper Limit 3200 72 2200 34 1600 30 1200 20 600 24 600 1.4
Female Lower Limit 1900 64 1300 29.5 600 24 500 14 300 11 300 0.9
Upper Limit 3200 72.5 2200 35.5 1100 33.5 1000 21 500 23 500 1.4
Adult Unisex Lower Limit 1346 49.0 796 23.0 401 13.4 243 7.0 133 6.0 115 0.69
Upper Limit 3779 80.0 2679 48.2 1451 41.0 1206 28.0 714 37.0 1009 2.83

Testing Laboratory

National University Hospital

Flow Cytometry Lab, 67724016 

Useful For

Clinical indication: For monitoring after immunosuppression, immune reconstitution following transplantation and screening for primary immunodeficiency.

This assay is designed to enumerate percent and absolute cell counts of lymphocyte subsets CD19 B-cells, CD3 T-cells (total, CD4, CD8) and CD56/CD16 NK-cells in whole blood.

Service Code

12675

Update

Last updated 25/03/2025

Test Info

This laboratory test has undergone in-house evaluation with appropriate quality assurance and control measures in line with laboratory best practice and accreditation requirements.

 

Reagent(s) used in this test is/are ordered through HSA’s Special Access Route, which provides interim access without full registration.

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