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Test Code CD48T Flow Cytometry - CD4/CD8 Ratio Panel

Important Note

Specimens are acceptable from Monday to Thursday.

Specimen must be RECEIVED by Laboratory before 11.00 AM on Tuesdays and Thursdays.

Do not send specimens on Fridays, Saturdays, Sundays, Public Holidays and after 11.00 AM on Tuesdays and Thursdays.

Laboratory Code

LAB5118

Specimen Requirements

Specimen Type: Whole Blood
Container/Tube: Lavender Top (EDTA)
Preferred Volume: 3.0 mL
Minimum Volume: 1.0 mL whole blood

Specimen Stability

Specimen Type Whole Blood
Ambient 48 hours
Refrigerate Not available
Frozen Not available

Panel Information

Panel includes the following tests:

  • Lymphocytes, Abs (cells/uL)
  • CD4 T Helper Cells (%)
  • CD4 T Helper Cells, Abs (cells/uL)
  • CD8 T Suppressor Cells (%)
  • CD8 T Suppressor Cells, Abs (cells/uL)
  • CD4/CD8 Ratio

Days and Times Performed

Tuesday and Thursday, TAT 7 - 9 days.

Specimen Condition

Haemolysis                            Not applicable
Icterus Not applicable
Lipaemia Not applicable
Others: Clotted or insufficient specimen Reject

Reference Interval

  Lymphocyte Absolute Count CD4 T Helper Cells CD4 T Helper Cells, Abs CD8 T Suppressor Cells CD8 T Suppressor Cells, Abs CD4/CD8 Ratio
Unit cells/uL % cells/uL % cells/uL Nil
At Birth Male Lower Limit 2000 36 800 13.5 400 1.9
Upper Limit 3900 48 1900 19.5 800 2.5
Female Lower Limit 1400 44 1000 18.5 400 1.7
Upper Limit 2900 53 1900 24.5 1000 3
2 days - 11 months old Male Lower Limit 2800 33 900 16 700 1.6
Upper Limit 5700 39 2300 24 1500 2.2
Female Lower Limit 4300 37 1700 16 800 1.9
Upper Limit 5700 48 2400 21 1100 2.9
1 - 6 years old Male Lower Limit 2500 29 1000 19 700 1.1
Upper Limit 5100 40 2100 25 1100 2
Female Lower Limit 3100 30 1000 20 700 1.2
Upper Limit 4700 40 1800 27 1200 2
7 - 18 years old Male Lower Limit 1500 27 600 23 500 1
Upper Limit 3200 34 1600 30 1200 1.4
Female Lower Limit 1900 29.5 600 24 500 0.9
Upper Limit 3200 35.5 1100 33.5 1000 1.4
Adult Unisex Lower Limit 1346 23.0 401 13.4 243 0.69
Upper Limit 3779 48.2 1451 41.0 1206 2.83

Testing Laboratory

National University Hospital

Flow Cytometry, 67724016 

Useful For

Determining over immunosuppression within the CD8 T-cell compartment, when used on transplant recipients and patients with autoimmune disorders receiving therapy with immunosuppressant agents.

Service Code

12674

Update

Last updated 25/03/2025

Test Info

This laboratory test has undergone in-house evaluation with appropriate quality assurance and control measures in line with laboratory best practice and accreditation requirements.

 

Reagent(s) used in this test is/are ordered through HSA’s Special Access Route, which provides interim access without full registration.

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