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Test Code LSTN Flow Cytometry - Lymphoma Screening Panel and Evaluation As Required

Important Note

Refer to FCM Flow Chart for ordering guide

Laboratory Code

LAB3052992

Specimen Requirements

Fresh specimen required. Submit one of the following:

(a) Specimen Type: Bone Marrow

      Container/Tube: Lavender Top (EDTA), Green Top (Sodium Heparin)

      Preferred Volume: 2.0 mL

 

(b) Specimen Type: Peripheral Blood

      Container/Tube: Lavender Top (EDTA), Green Top (Sodium Heparin)

      Preferred Volume: 3.0 mL

      Collection Instructions: Collect 5.0 - 6.0 mL blood if WBC < 5 x 109/L.

     

(c) Specimen Type: Lymph Node / Solid Tumour

     Container/Tube: Sterile container with 5.0 mL RPMI 1640 media

     Preferred Volume: 5 mm(3) or larger biopsy

     Collection Instructions: Place tissue directly into 5.0 mL RPMI 1640 media after biopsy.

     Do not leave tissue to air-dry or fix tissue in formalin.

     (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

     

(d) Specimen Type: Body Fluid

      Container/Tube: Sterile tube with 0.6 mL transfix media

      Preferred Volume: 3.0 - 6.0 mL

      (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

 

(e) Specimen Type: "Small cell number" samples, e.g. CSF, vitreous biopsies

      Container/Tube: Sterile tube with 0.2 mL transfix media

      Preferred Volume: 1.0 - 2.0 mL

     (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

 

(f) Specimen Type: Fine Needle Aspirate

     Container/Tube: Sterile container with 5.0 mL RPMI 1640 media

     Collection Instructions: Place fine needle aspirate directly into 5.0 mL RPMI 1640 media after           collection.

     Do not place in formalin or in a sterile container without any tissue culture medium.

    (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

 

Additional Collection Instructions: All specimens should be shipped to the lab in ambient temperature (18 - 25oC) within 24 - 48 hrs of collection. Specimen received after 48 hrs of collection will nonetheless be processed but results may be compromised. For overseas specimens, avoid shipping on Friday or eve of public holidays.

Specimen Stability

Fresh specimen required

Ambient 24 hours (preferred)
Refrigerate 48 hours       

Methodology

Flow Cytometry

Days and Times Performed

Monday through Friday. TAT 14 - 16 days (STAT 7 - 9 days)

Reference Interval

See laboratory report

Testing Laboratory

National University Hospital
Flow Cytometry, 67724016

Useful For

This test is used to diagnose new case lymphoma, determine subtype of lymphoma, and for subsequent MRD monitoring. The upfront cost of the order is charged. Flow cytometry lab will extend to full evaluation according to abnormality found.

Service Code

Upfront charge codes: 8LST (12651) + 8BCLP (12658)

Final charge codes will be determined and rectified based on final tests performed as required.

Update

Last updated 22/01/2025

Test Info

Pre-arrange with Haematology before ordering the test.

 

This laboratory test has undergone in-house evaluation with appropriate quality assurance and control measures in line with laboratory best practice and accreditation requirements.

 

Reagent(s) used in this test is/are ordered through HSA’s Special Access Route, which provides interim access without full registration.

Unacceptable Condition

Specimen Type
Bone Marrow/Peripheral Blood/Body Fluid Frozen
Lymph Node/Solid Tumour/Other Tissues Frozen or air-dry or formalin fixed tissues

Reflex Tests

Once specimen is collected:

  • 8LST
  • 8BCLP

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