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Test Code 8LST Flow Cytometry - Lymphoma Screening Panel

Important Note

**Refer to FCM Flow Chart for ordering guide**

Specimen Requirements

Submit one of the following:

 

Fresh specimen required

 

(a) Specimen Type: Bone marrow

      Container/Tube: Lavender Top (EDTA), Green Top (Sodium Heparin)

      Volume: 2mL

 

(b) Specimen Type: Peripheral blood

      Container/Tube: Lavender Top (EDTA), Green Top (Sodium Heparin)

      Volume: 3mL

      Collection Instructions: Collect 5-6mL blood if WBC < 5 x 109/L.

     

(c) Specimen Type: Lymph Node / Solid Tumour

      Container/Tube: Sterile container with 5mL RPMI 1640 media

      Volume: 5mm(3) or larger biopsy

       Collection Instructions: Place tissue directly into 5mL RPMI 1640 media after biopsy. Do not leave tissue

        to air-dry or fix tissue in formalin.

        (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

     

(d) Specimen Type: Body fluid

      Container/Tube: Sterile tube with 0.6mL transfix media

       Volume: 3-6mL

        (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

 

(e) Specimen Type: "Small cell number" samples, e.g. CSF, vitreous biopsies

       Container/Tube: Sterile tube with 0.2mL transfix media

        Volume: 1-2mL

       (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

 

(f) Specimen Type: Fine needle aspirate

      Container/Tube: Sterile container with 5mL RPMI 1640 media

      Collection Instructions: Place fine needle aspirate directly into 5mL RPMI 1640 media after collection. Do

      not place in formalin or in a sterile container without any tissue culture medium.

      (Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)

 

Additional Collection Instructions: All specimens should be shipped to the lab in ambient temperature (18-25oC) within 24-48 hours of collection. Specimen received after 48 hours of collection will nonetheless be processed but results may be compromised. For overseas specimens, avoid shipping on Friday or eve of public holidays.

Reference Interval

See laboratory report

Days and Times Performed

Monday through Friday. 20 working days (STAT 5 days)

Methodology

Flow Cytometry

Service Code

12651

Update

Last updated 22/10/2024

Added new test info

Unacceptable Condition

 

Specimen Type
Bone Marrow/Peripheral Blood/Body Fluid Frozen
Lymph Node/Solid Tumour/Other Tissues Frozen or air-dry or formalin fixed tissues

 

Specimen Stability

** Preferred temperatures for storage prior to and during shipping to NUH **

 

Fresh specimen required

Ambient (Preferred) 24 hours
Refrigerate 48 hours                   

 

Testing Laboratory

National University Hospital
Flow Cytometry, 67724016

Useful For

To determine clonality of lymphoid cells and diagnose new case lymphoma.

Test Info

Pre-arrange with Haematology before ordering the test.

 

This laboratory test has undergone in-house evaluation with appropriate quality assurance and control measures in line with laboratory best practice and accreditation requirements.

 

Reagent(s) used in this test is/are ordered through HSA’s Special Access Route, which provides interim access without full registration.

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