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HelpTest Code 8AML6 Flow Cytometry - AML Follow-up Full Panel
Specimen Requirements
Submit one of the following:
Fresh specimen required
(a) Specimen Type: Bone marrow
Container/Tube: Lavender Top (EDTA), Green Top (Sodium Heparin)
Volume: 2mL
(b) Specimen Type: Peripheral blood
Container/Tube: Lavender Top (EDTA), Green Top (Sodium Heparin)
Volume: 3mL
Collection Instructions: Collect 5-6mL blood if WBC < 5 x 109/L.
(c) Specimen Type: Lymph Node / Solid Tumour
Container/Tube: Sterile container with 5mL RPMI 1640 media
Volume: 5mm(3) or larger biopsy
Collection Instructions: Place tissue directly into 5mL RPMI 1640 media after biopsy. Do not leave tissue
to air-dry or fix tissue in formalin.
(Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)
(d) Specimen Type: Body fluid
Container/Tube: Sterile tube with 0.6mL transfix media
Volume: 3-6mL
(Prearrange with Flow Cytometry Laboratory for transport media prior sample collection)
Additional Collection Instructions: All specimens should be shipped to the lab in ambient temperature (18-25oC) within 24-48 hours of collection. Specimen received after 48 hours of collection will nonetheless be processed but results may be compromised. For overseas specimens, avoid shipping on Friday or eve of public holidays.
Reference Interval
See laboratory report
Days and Times Performed
Monday through Friday. 20 working days (STAT 5 days)
Methodology
Flow Cytometry
Service Code
12668
Update
Last updated 22/10/2024
Added new test info
Unacceptable Condition
| Specimen Type | |
|---|---|
| Bone Marrow/Peripheral Blood/Body Fluid | Frozen |
| Lymph Node/Solid Tumour/Other Tissues | Frozen or air-dry or formalin fixed tissues |
Specimen Stability
** Preferred temperatures for storage prior to and during shipping to NUH **
Fresh specimen required
| Ambient (Preferred) | 24 hours |
|---|---|
| Refrigerate | 48 hours |
Testing Laboratory
National University Hospital
Flow Cytometry, 67724016
Useful For
The panel is used for follow-up examination of acute promyelocytic leukaemia, acute megakaryocytic leukaemia, acute basophilic leukaemia, blastic plasmacytoid dendritic cell neoplasms and some ambiguous AML cases. The previously detected characteristic phenotypic markers and expression pattern are used for MRD monitoring.
Test Info
Pre-arrange with Haematology before ordering the test.
This laboratory test has undergone in-house evaluation with appropriate quality assurance and control measures in line with laboratory best practice and accreditation requirements.
Reagent(s) used in this test is/are ordered through HSA’s Special Access Route, which provides interim access without full registration.