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Test Code TES Testosterone

Laboratory Code

LAB124

Specimen Requirements

Specimen Type: Serum
Container/Tube: Gold Top (SSTII) 
Volume: 5.0 mL 
Minimum Volume: 0.5 mL serum
Collection Instructions: Allow specimen to clot for 30 mins and centrifuge within 2 hrs.

Specimen Stability

Specimen Type Serum
Ambient 8 hours
Refrigerate 7 days
Frozen 6 months 

Methodology

Chemiluminescent Immunoassay

Days and Times Performed

24 hrs, TAT 1 day

Specimen Condition

Haemolysis Alert/Reject
Icterus OK
Gross Lipaemia Alert/Reject
Others Not applicable

Reference Interval

Age Male
4 days – 5 mths 0.3 – 10.4 nmol/L
6 mths – 8 yrs ≤ 1.2 nmol/L
9 – 10 yrs ≤ 0.8 nmol/L
11 – 13 yrs ≤ 15.4 nmol/L
14 – 15 yrs 1.3 – 21.9 nmol/L
16 – 18 yrs 5.1 – 27.6 nmol/L
19 – 49 yrs 8.3 – 30.2 nmol/L
≥ 50 yrs 7.7 – 24.8 nmol/L

 

Age Female
4 days – 8 yrs ≤ 2.2 nmol/L
9 – 12 yrs ≤ 1.0 nmol/L
13 – 14 yrs 0.4 – 1.5 nmol/L
15 – 18 yrs 0.5 – 1.7 nmol/L
19 – 49 yrs 0.5 – 1.9 nmol/L
≥ 50 yrs 0.4 – 1.2 nmol/L

 

Male 
Tanner Stage I: ≤ 1.0 nmol/L 
Tanner Stage II: 0.1 – 9.7 nmol/L 
Tanner Stage III: 0.3 – 22.7 nmol/L 
Tanner Stage IV: 0.7 – 26.2 nmol/L 
Tanner Stage V: 0.6 – 31.3 nmol/L

 

Female 
Tanner Stage I: ≤ 1.1 nmol/L 
Tanner Stage II: 0.7 nmol/L 
Tanner Stage III: 0.3 – 1.4 nmol/L 
Tanner Stage IV: 0.5 – 1.9 nmol/L 
Tanner Stage V: 0.6 – 3.4 nmol/L

 

Note:

Nandrolone may lead to elevated testosterone levels by > 300% when 
tested at 30 nmol/L concentration, according to manufacturer’s guide.

 

17-ketotestosterone may lead to reduced testosterone by < 10% when 
tested at 5 nmol/L concentration, according to manufacturer’s guide.

Testing Laboratory

National University Hospital

Clinical Chemistry, 67724346

Useful For

Diagnosis of hypogonadism in males.

Investigation of hirsutism and virilisation in females.

Service Code

31663

LOINC Code Information

83115-6

Update

Last updated 13/03/2025