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Test Code TB4 TB Quantiferon

Laboratory Code

LAB5105

Specimen Requirements

Specimen Type: Whole Blood
Container/Tube: Green Top (Li Heparin)
Volume: 4.0 mL tube (2 full tubes)
Minimum Volume: Whole blood, 5.0 mL (2 full tubes)

Collection Instructions: 

Kindly ensure specimen reaches NUH Laboratory by 3pm on the same day of collection on Mondays to Thursdays ONLY. Specimens will not be accepted on Fridays, Saturdays, Sundays, eve of public holidays and on public holidays. 

Specimen Stability

Specimen Type Whole Blood
Ambient 16 hours 
Refrigerate Not available
Frozen Not available

Methodology

Enzyme Immunoassay

Days and Times Performed

Friday, TAT 2 - 8 days

Specimen Condition

Haemolysis OK
Icterus OK
Lipaemia OK
Others: Samples that arrive at laboratory > 16 hours after collection. Samples dispatched on eve of Public Holiday, Saturday and Sunday. Reject 

Reference Interval

Negative

Testing Laboratory

National University Hospital
Clinical Chemistry, 67724346

Useful For

It can be used as an aid in diagnosing Mycobacterium tuberculosis infection, including both latent tuberculosis infection (LTBI) and tuberculosis (TB) disease. This enzyme-linked immunosorbent assay test detects the release of interferon-gamma in fresh heparinized whole blood from sensitized persons when it is incubated with mixtures of synthetic peptides simulating two proteins present in M. tuberculosis: early secretory antigenic target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10). ESAT-6 and CFP-10 are secreted by all M. tuberculosis and pathogenic M. bovis strains. These proteins are absent from all Bacille Calmette-Guerin (BCG) vaccine strains and from commonly encountered non-tuberculous mycobacteria (NTM).

Service Code

32148

LOINC Code Information

QFTC 39017-9
nil1r 88518-6
tb1nr 64084-7
tb2nr 88517-8
mitnr 71774-4

Alias

Interferon-gamma

QuantiFERON-TB Gold Plus

Update

Last updated 21/02/2025