Test Code PHNB Phenobarbital
Laboratory Code
LAB30
Specimen Requirements
Submit only one of the following:
Preferred
Specimen Type: Serum
Container/Tube: Gold Top (SSTII)
Volume: 5.0 mL
Minimum Volume: 0.5 mL serum
Collection Instructions: Allow specimen to clot for 30 mins and centrifuge within 2 hrs.
Acceptable
Specimen Type: Plasma
Container/Tube: Green Top (Li Heparin/PST)
Volume: 4.5 mL
Minimum Volume: 0.5 mL plasma
Collection Instructions: Centrifuge within 2 hrs.
Specimen Stability
Specimen Type | Serum/Plasma |
---|---|
Ambient | 7 days |
Refrigerate | 6 months |
Frozen | 6 months |
Methodology
Particle-Enhanced Turbidimetric Inhibition Immunoassay (PETINIA)
Days and Times Performed
24 hrs, TAT 1 day
Specimen Condition
Haemolysis | OK |
---|---|
Icterus | OK |
Lipaemia | OK |
Others | Not applicable |
Reference Interval
The desired therapeutic effect is usually achieved in the serum concentration range of 15 to 40 mg/L. Concentrations of 35 to 80 mg/L are associated with slowness, ataxia, and nystagmus.
Concentrations of 65 to 117 mg/L are associated with coma with reflexes.
Concentrations > 100 mg/L are associated with coma without reflexes.
Critical value: > 40 mg/L
Testing Laboratory
National University Hospital
Clinical Chemistry, 67724346
Useful For
Therapeutic drug monitoring.
Service Code
31821
LOINC Code Information
3948-7
Alias
Phenobarbitone
Update
Last updated 12/03/2025