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Test Code Methotrexate Methotrexate 

Important Note

Wrap specimen in aluminium foil to protect from light.

Additional Codes

Epic Ordering Code LAB481
NBS code LBC08017
Service code 31819

Specimen Requirements

Submit the following:

Container:
Dark Green Top (Li Heparin)

Additional information: Protect sample from light with aluminium foil and send to the Laboratory immediately.

Processing Requirements

Centrifuge within 2 hours.

Specimen type: Plasma

Volume: 0.5 mL

Additional information: For specimens collected outside NUH, aliquot the plasma into a secondary tube. Protect the specimen from light with aluminium foil, and send it frozen to the laboratory.

Specimen Stability

Specimen Type Plasma
Ambient 1 day
Refrigerate 2 days
Frozen 6 months (Preferred)

Specimen Condition

Haemolysis OK
Icterus OK
Lipaemia OK
Others: Specimens not protected from light Reject     

Methodology

Chemiluminescent Immunoassay

Days and Times Performed

24 hrs, TAT 1 day

Reference Interval

Post treatment 5.00 – 10.00 umol/L
24 – 48 hrs 0.50 – 1.00 umol/L
48 – 72 hrs < 0.20 umol/L

Testing Laboratory

National University Hospital
Clinical Chemistry, 67724346

Useful For

Therapeutic drug monitoring.

Update

Last updated 26/07/2026

LOINC Code Information

14836-1