Test Code MRACF Culture and Sensitivity, Respiratory, Cystic Fibrosis
Specimen Requirements
Submit only one of the following
Specimen Type: Sputum, induced sputum, ETT
aspirate, tracheal aspirate, BAL
Container/Tube: Sterile screw-capped container
Volume: 2.0mL
Minimum Volume: 0.5mL
Collection Instructions:
Sputum:
- Rinse mouth with water to remove food particles.
- Collect 4 to 5 deep coughs into a sterile container.
- Do not send saliva.
- Cap container tightly, label, and send to the laboratory immediately.
BAL: BAL fluid is preferred to bronchial washings for bacterial
culture.
Specimen Type: Throat swab. ("Cough swab" from
paediatric patients who are unable to expectorate sputum)
Container/Tube: eSwab, M40 transystem Amies agar
transport media (Copan)(for non-NUH patients)
Volume: 1 swab
Collection Instructions: Please refer to Test Catalogue -> Test Collection Information -> eSwab Training Slides
Please indicate if extended incubation is required when slow
growing bacteria such as Nocardia, Actinomyces are suspected.
For non-CF patient, please use the test code "Culture and Sensitivity, Respiratory" for routine culture.
Sample Stability
** Preferred temperatures for storage prior to and during shipping to NUH **
Specimen Type: | All types |
---|---|
Ambient: | Submit sample to lab immediately |
Refrigerate: | Unacceptable |
Frozen: | Unacceptable |
Reference Interval
No growth of respiratory pathogens.
Days and Times Performed
Monday through Sunday, TAT 4-6 days
Methodology
Conventional Culture
Service Code
23062
Update
Last updated 11/10/2021
Unacceptable Condition
Frozen sample.
Testing Laboratory
National University Hospital
Clinical Microbiology, 67724343
Useful For
Isolation of microorganisms associated with cystic fibrosis.
Test Information
A Gram smear is included with this test.