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Test Code MRACF Culture and Sensitivity, Respiratory, Cystic Fibrosis

Laboratory Code

LAB3053104

Specimen Requirements

Submit only one of the following

 

Specimen Type: Sputum, induced sputum, ETT aspirate, tracheal aspirate, BAL
Container/Tube: Sterile screw-capped container
Volume: 2.0 mL
Minimum Volume: 0.5 mL
Collection Instructions:

 

Sputum:

  • Rinse mouth with water to remove food particles.
  • Collect 4 to 5 deep coughs into a sterile container.
  • Do not send saliva.
  • Cap container tightly, label, and send to the laboratory immediately.

BAL: BAL fluid is preferred to bronchial washings for bacterial culture.
 

Specimen Type: Throat swab. ("Cough swab" from paediatric patients who are unable to expectorate sputum)
Container/Tube: eSwab, M40 transystem Amies agar transport media (for non-NUH patients)
Volume: 1 swab

Collection Instructions: Please refer to eSwab Training Slides.

 

Please indicate if extended incubation is required when slow growing bacteria such as Nocardia, Actinomyces are suspected.
 

For non-CF patient, please use the test code "Culture and Sensitivity, Respiratory" for routine culture.

Specimen Stability

Specimen Type All types
Ambient Submit sample to lab immediately
Refrigerate Unacceptable
Frozen Unacceptable

Methodology

Conventional Culture

Days and Times Performed

Monday through Sunday, TAT 4 - 6 days

Reference Interval

No growth of respiratory pathogens.

Testing Laboratory

National University Hospital

Clinical Microbiology, 67724343

Useful For

Isolation of microorganisms associated with cystic fibrosis.

Service Code

23062

LOINC Code Information

79425-5

Update

Last updated 17/3/2025

Unacceptable Condition

Frozen sample.

Test Information

A Gram smear is included with this test.