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HelpTest Code IHA Factor VIII or Factor IX Inhibitor Assay
Specimen Requirements
Submit only one of the following specimens
Specimen Type: Whole Blood
Container/Tube: 3 Light Blue Top (3.2% Sodium
Citrate)
Volume: 3 × 2.7mL Whole Blood (Tubes
must be full)
Collection Instructions: Send whole blood
specimens to laboratory immediately.
Specimen Type: Platelet Poor Plasma
Container/Tube: 3 Light Blue Top (3.2% Sodium
Citrate)
Submission Container/Tube:
Plastic Vial
Minimum Volume: 3.0mL Plasma
Collection Instructions:
1. Light blue top tube must be properly filled with whole
blood.
2. Whole blood specimen should be double centrifuged and plasma
separated from cells within 1 hour of collection.
3. Plasma should be separated into 2 aliquots with minimum
volume of 1.5mL each.
4. Freeze the aliquots in well-closed plastic tubes
immediately.
Reference Interval
< 0.5 Bethesda Unit
Days and Times Performed
Tuesday through Friday, TAT 10 working days
Methodology
Photo-optical Clot Detection, Bethesda Assay
Service Code
12413
Update
Last updated 25/02/2020
Unacceptable Condition
| Haemolysis | Gross reject |
|---|---|
| Icterus | Gross reject |
| Lipaemic | Gross reject |
| Others | Clotted or insufficient reject |
Specimen Stability
**Preferred temperatures for storage prior to and during shipping to NUH**
| Specimen Type | Plasma |
|---|---|
| Ambient | 4 hours |
| Refrigerate | 4 hours |
| Frozen | 14 days |
Testing Laboratory
National University Hospital
Haematology, 67725320
Useful For
Detecting the presence and titer of a specific factor inhibitor directed against coagulation Factor VIII or Factor IX.
Alias
Factor VIII Inhibitor
Factor IX Inhibitor
Bethesda Assay