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Test Code IHA Factor VIII or Factor IX Inhibitor Assay

Important Note

Please consult haematologist before ordering this test.

To specify request for Factor VIII or Factor IX inhibitor assay.

Additional Codes

Epic Ordering Code LAB1116 (FVIII) / LAB3040775 (FIX)
NBS code LBC00375
Service code 12413

Specimen Requirements

Submit the following: 

 

Preferred:
Light Blue Top

Processing Requirements

Submit only one of the following specimens


Specimen Type: Whole Blood
Container/Tube: 3 Light Blue Top (3.2% Sodium Citrate)
Volume: 3 × 2.7 mL Whole Blood (Tubes must be full)
Collection Instructions: Send whole blood specimens to laboratory immediately.


Specimen Type: Platelet Poor Plasma
Container/Tube: 3 Light Blue Top (3.2% Sodium Citrate)
Submission Container/Tube: Plastic Vial

Minimum Volume: 3.0 mL plasma
Collection Instructions:
1. Light blue top tube must be properly filled with whole blood.

2. Whole blood specimen should be double centrifuged and plasma separated from cells within 1 hr of collection.
3. Plasma should be separated into 2 aliquots with minimum volume of 1.5 mL each.
4. Freeze the aliquots in well-closed plastic tubes immediately.

Specimen Stability

Specimen Type Plasma
Ambient 4 hours
Refrigerate 4 hours
Frozen 14 days (preferred)

Specimen Condition

Haemolysis Gross reject
Icterus Gross reject
Lipaemia Gross reject
Others Clotted or insufficient reject

Methodology

Photo-optical Clot Detection, Bethesda Assay

Days and Times Performed

Monday through Friday, TAT 7 days

Reference Interval

< 0.5 Bethesda Unit

Testing Laboratory

National University Hospital
Haematology, 67725320

Useful For

Detecting the presence and titer of a specific factor inhibitor directed against coagulation Factor VIII or Factor IX.

Update

Last updated 22/06/2026

LOINC Code Information

13591-3

Alias

Bethesda Assay

Factor VIII Inhibitor
Factor IX Inhibitor