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Test Code HIVGR HIV-1 RNA Reverse Transcriptase & Protease & Integrase Genotypic Resistance

Important Note

Genotypic resistance testing requires a minimum viral load to be successful. Minimum HIV viral load > 2,000 copies/ml.

NUH Laboratory strongly recommends ordering viral load (VL) testing (Test code 1033057 HIVL1) together with genotypic resistance testing. If viral load is below the acceptable threshold, genotypic testing will not be performed, and the genotypic test will be cancelled with refund.

If genotypic resistance testing is ordered without a concurrent viral load, NUH recommends that a documented viral load within the preceding 14 days is provided with the test order.

In the absence of a concurrent viral load, testing will proceed at the requester’s risk.

If genotypic testing fails due to insufficient viral load, the result will be reported as unsuccessful, no reflex viral load testing will be performed.

Laboratory Code

LAB2203

Specimen Requirements

Preferred:
Specimen Type: Whole Blood
Container/Tube: Lavender Top (EDTA)
Specimen Volume: 3.0 mL x 4 tubes

Acceptable:
Specimen Type: Plasma
Container/Tube: Sterile Container
Specimen Volume: 4.0 mL

Specimen Stability

Whole Blood (EDTA) 24 hours, ambient/refrigerated (2 - 25 °C)
Plasma Frozen at -20 to -80 °C

Methodology

Reverse Transcription Polymerase Chain Reaction, High-Throughput Sequencing

Days and Times Performed

TAT 21 - 30 days

Specimen Condition

Whole Blood: Frozen or hemolysis Reject

Reference Interval

An interpretative report will be provided.

Testing Laboratory

National University Hospital
Molecular Diagnosis Centre, 67724384

Useful For

Identification of HIV-1 drug resistance mutations (DRM) associated with resistance to protease inhibitors, nucleotide reverse transcriptase inhibitors, non-nucleotide reverse transcriptase inhibitors, and integrase inhibitors.

Service Code

1033068

LOINC Code Information

80689-3

Update

Last updated 05/02/2026

Test Info

(a)  This test was developed by NUH Laboratory Medicine.

(b) This laboratory test has undergone in-house evaluation with appropriate quality assurance and control measures in line with laboratory best practice and accreditation requirements. 

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