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Test Code HBFK Kleihauer Test

Laboratory Code

LAB3040610

Specimen Requirements

Specimen Type: Whole Blood
Container/Tube: Lavender Top (EDTA)
Volume: 3.0 mL

Minimum Volume: 1.5 mL

Collection Instructions:

1. Do not centrifuge.

2. Send whole blood specimen to laboratory immediately.

Specimen Stability

Specimen Type Whole Blood
Ambient 6 hours (preferred)
Refrigerate 3 days
Frozen Not available

Methodology

Kleihauer-Betke Method, Acid Elution

Days and Times Performed

24 hrs, TAT 1 day

Specimen Condition

Haemolysis Reject
Icterus Reject
Lipaemia Reject
Others: Clotted or insufficient specimen Reject

Reference Interval

No fetal cells seen

Testing Laboratory

National University Hospital
Haematology, 67725354

Useful For

Used to determine fetomaternal haemorrhage by assessing amount of fetal haemoglobin present in mother’s blood and is usually performed on Rhesus negative mother to determine dose of Rh immune prophylaxis required. It may be helpful to estimate fetomaternal haemorrhage in Rhesus positive women who have had an intrauterine death or stillbirth if the cause of death is unknown or fetal haemorrhage is suspected.

Service Code

12309

LOINC Code Information

75373-1

Update

Last updated 20/03/2025