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HelpEstradiol, Serum
Reporting Name
Estradiol, Mass Spectrometry, SNote: This is Estradiol, high sensitive
For NUH patients - use Epic to order (LAB3053161)
NUH service code - 36964
Mayo test code - EEST
Useful For
All applications that require moderately sensitive measurement of estradiol:
-Evaluation of hypogonadism and oligo-amenorrhea in females
-Assessing ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)
-In conjunction with luteinizing hormone measurements, monitoring of estrogen replacement therapy in hypogonadal premenopausal women
-Evaluation of feminization, including gynecomastia, in males
-Diagnosis of estrogen-producing neoplasms in males and, to a lesser degree, females
-As part of the diagnosis and workup of precocious and delayed puberty in females, and, to a lesser degree, males
-As part of the diagnosis and workup of suspected disorders of sex steroid metabolism (eg, aromatase deficiency and 17 alpha-hydroxylase deficiency)
-As an adjunct to clinical assessment, imaging studies and bone mineral density measurement in the fracture risk assessment of postmenopausal women, and, to a lesser degree, older men
-Monitoring low-dose female hormone replacement therapy in postmenopausal women
-Monitoring antiestrogen therapy (eg, aromatase inhibitor therapy)
Testing Algorithm
For more information see Steroid Pathways.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Serum RedSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (SST/serum gel tubes are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.2 mL
Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Additional Information: For more information see Steroid Pathways.
Specimen Minimum Volume
0.7 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days | |
| Ambient | 28 days | ||
| Frozen | 28 days |
Special Instructions
Reference Values
CHILDREN*
1 to14 days: Estradiol levels in newborns are very elevated at birth but will fall to prepubertal levels within a few days.
Males
|
Tanner stages# |
Mean age |
Reference range |
|
Stage I (>14 days and prepubertal) |
7.1 years |
Undetectable-13 pg/mL |
|
Stage II |
12.1 years |
Undetectable-16 pg/mL |
|
Stage III |
13.6 years |
Undetectable-26 pg/mL |
|
Stage IV |
15.1 years |
Undetectable-38 pg/mL |
|
Stage V |
18 years |
10-40 pg/mL |
#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (± 2) years. For boys, there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
Females
|
Tanner stages# |
Mean age |
Reference range |
|
Stage I (>14 days and prepubertal) |
7.1 years |
Undetectable-20 pg/mL |
|
Stage II |
10.5 years |
Undetectable-24 pg/mL |
|
Stage III |
11.6 years |
Undetectable-60 pg/mL |
|
Stage IV |
12.3 years |
15-85 pg/mL |
|
Stage V |
14.5 years |
15-350 pg/mL** |
#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (± 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
*The reference ranges for children are based on the published literature(1,2), cross-correlation of our assay with assays used to generate the literature data, and on our data for young adults.
ADULTS
Males: 10-40 pg/mL
Females
Premenopausal: 15-350 pg/mL**
Postmenopausal: <10 pg/mL
**E2 levels vary widely through the menstrual cycle.
Conversion factor
E2: pg/mL x 3.676=pmol/L (molecular weight=272)
For International System of Units (SI) conversion of Reference Values see https://www.mayocliniclabs.com/order-tests/si-unit-conversion.html
Day(s) Performed
Monday through Saturday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82670
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| EEST | Estradiol, Mass Spectrometry, S | 2243-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 81816 | Estradiol, Mass Spectrometry, S | 2243-4 |
Report Available
2 to 6 daysReject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
Updated on 21/03/2022