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HelpTest Code E2 Estradiol (E2)
Laboratory Code
LAB523
Specimen Requirements
Specimen Type: Serum
Container/Tube: Gold Top (SSTII)
Volume: 5.0 mL
Minimum Volume: 0.5 mL serum
Collection Instructions: Allow specimen to clot for 30 mins and centrifuge within 2 hrs.
Specimen Stability
| Specimen Type | Serum |
|---|---|
| Ambient | 24 hours |
| Refrigerate | 7 days |
| Frozen | 6 months |
Methodology
Chemiluminescent Immunoassay
Days and Times Performed
24 hrs, TAT 1 day
Specimen Condition
| Gross Haemolysis | Alert/Reject |
|---|---|
| Icterus | OK |
| Gross Lipaemia | Alert/Reject |
| Others | Not applicable |
Reference Interval
| Age | Female |
|---|---|
| 15 days – < 1 yr | ≤ 92 pmol/L |
| 9 – 10 yrs | ≤ 176 pmol/L |
| 11 – < 12 yrs | ≤ 345 pmol/L |
| 12 – 13 yrs | ≤ 631 pmol/L |
| 14 – 18 yrs | ≤ 936 pmol/L |
| Age | Male |
|---|---|
| 15 days – < 1 yr | ≤ 92 pmol/L |
| 11 – 12 yrs | ≤ 95 pmol/L |
| 13 – 14 yrs | ≤ 102 pmol/L |
| 15 – 18 yrs | ≤ 142 pmol/L |
| ≥ 19 yrs | ≤ 191 pmol/L |
Normal Menstruating Females:
Follicular Phase: ≤ 921 pmol/L
Mid-Cycle Phase: 139 – 2382 pmol/L
Luteal Phase: ≤ 1145 pmol/L
Postmenopausal Females not on HRT: ≤ 103 pmol/L
Postmenopausal Females on HRT: ≤ 528 pmol/L
HRT: Hormone Replacement Therapy
Female
Tanner Stage I: Not available
Tanner Stage II: ≤ 96 pmol/L
Tanner Stage III: ≤ 317 pmol/L
Tanner Stage IV: ≤ 517 pmol/L
Tanner Stage V: ≤ 762 pmol/L
Male
Tanner Stage I: Not available
Tanner Stage II: Not available
Tanner Stage III: Not available
Tanner Stage IV: ≤ 128 pmol/L
Tanner Stage V: ≤ 126 pmol/L
This Estradiol assay should not be used to assess Estradiol levels for patients undergoing Fulvestrant or Mifepristone treatment.
There is potential for cross reactivity with medications which inhibit tumour cell proliferation (e.g. CDK 4/6 inhibitors) and medications that activate or interfere with production of steroid hormones (e.g. Aromatase inhibitors).
In such cases, an alternate method such as mass spectrometry method may be used.
Testing Laboratory
National University Hospital
Clinical Chemistry, 67724346
Useful For
Female: Assessment of hypothalamic-pituitary ovarian axis.
Male: Investigation of unexplained gynaecomastia.
Service Code
31666
LOINC Code Information
83097-6
Update
Last updated 11/03/2025