Sign in →

Test Code E2 Estradiol (E2)

Specimen Requirements

Specimen Type: Serum
Container/Tube: Gold Top (SSTII) 
Volume: 5.0mL
Minimum Volume: 0.5mL serum
Collection Instructions: Allow specimen to clot for 30 minutes and centrifuge within 2 hours

Sample Stability

** Preferred temperatures for storage prior to and during shipping to NUH **

 

Specimen Type: Serum
Ambient: Send the sample to the laboratory immediately
Refrigerate: 7 days
Frozen: 6 months

 

Reference Interval

Age Female
15 days - < 1 yr ≤92 pmol/L
9 - 10 yrs  ≤176 pmol/L
11 - <12 yrs  ≤345 pmol/L
12 - 13 yrs ≤631 pmol/L
14 - 18 yrs ≤936 pmol/L

 

Age Male
15 days - < 1yr ≤92 pmol/L
11 - 12 yrs ≤95 pmol/L
13 - 14 yrs ≤102 pmol/L
15 - 18 yrs ≤142 pmol/L
≥19 yrs ≤191 pmol/L

 

 

Normal Menstruating Females: 
Follicular Phase: ≤ 921 pmol/L 
Mid-Cycle Phase: 139 - 2382 pmol/L 
Luteal Phase: ≤ 1145 pmol/L 
Postmenopausal Females not on HRT: ≤ 103 pmol/L 
Postmenopausal Females on HRT: ≤ 528 pmol/L 
HRT: Hormone Replacement Therapy

 

Female 
Tanner Stage I: Not Available 
Tanner Stage II: ≤ 96 pmol/L 
Tanner Stage III: ≤ 317 pmol/L 
Tanner Stage IV: ≤ 517 pmol/L 
Tanner Stage V: ≤ 762 pmol/L

 

Male 
Tanner Stage I: Not Available 
Tanner Stage II: Not Available 
Tanner Stage III: Not Available 
Tanner Stage IV: ≤ 128 pmol/L 
Tanner Stage V: ≤ 126 pmol/L 

 

This Estradiol assay should not be used to assess Estradiol levels for patients undergoing Fulvestrant or Mifepristone treatment.

 

There is potential for cross reactivity with medications which inhibit tumour cell proliferation (e.g. CDK 4/6 inhibitors) and medications that activate or interfere with production of steroid hormones (e.g. Aromatase inhibitors).

 

In such cases, an alternate method such as mass spectrometry method may be used.
 

Days and Times Performed

24 hours, TAT 1 day

Methodology

Chemiluminescent Immunoassay

Service Code

31666

Update

last updated 16/05/2024

Updated on Methodology.

 

Unacceptable Condition

 

Haemolysis NA
Icterus NA
Lipaemic NA
Others NA

 

Testing Laboratory

National University Hospital

Clinical Chemistry, 67724346

Useful For

Female: Assessment of hypothalamic-pituitary ovarian axis.

Male: Investigation of unexplained gynaecomastia.