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HelpTest Code DHVD 1,25-Dihydroxyvitamin D, Serum
Additional Codes
| Epic Ordering Code | LAB2218 |
| NBS code | LBC00002 |
| Service code | 36900 |
Reporting Name
1,25-Dihydroxyvitamin D, SSpecimen Type
SerumOrdering Guidance
The 25-hydroxyvitamin D test (25HDN / 25-Hydroxyvitamin D2 and D3, Serum) in serum is the preferred initial test for assessing vitamin D status and most accurately reflects the body's vitamin D stores. In the presence of renal disease or hypercalcemia, testing of 1,25-dihydroxy vitamin D (DHVD) may be needed to adequately assess vitamin D status.
Specimen Required
Patient Preparation:
Fasting: 4 hours, preferred but not required
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL Serum
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
NUH Outreach Laboratories:
Specimen Requirements
Submit only one of the following:
Preferred: Red Top
Acceptable: Yellow Top
Fasting: 4 hours, preferred but not required
Processing Requirements
Centrifuge and aliquot serum into a Mayo plastic tube.
Specimen type: Serum
Volume: 1.5 mL
Storage/transport requirements: Frozen
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
-Renal Diagnostics Test Request (T830)
Specimen Minimum Volume
Serum: 0.7 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 30 days |
| Frozen | 30 days | |
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Day(s) Performed
Monday through Friday
Report Available
2 to 5 daysPlease note that this is a referral test requiring transport to the external testing facility and an additional 3-5 days is required.
Reference Values
Males:
<16 years: 24-86 pg/mL
≥16 years: 18-64 pg/mL
Females:
<16 years: 24-86 pg/mL
≥16 years: 18-78 pg/mL
For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Evaluation of selected patients with disorders of calcium and phosphate metabolism
Evaluation of chronic kidney disease with suspected abnormalities of vitamin D metabolism
Investigation of suspected hereditary disorders of vitamin D metabolism, including vitamin D-dependent rickets
Evaluation of parathyroid hormone-independent hypercalcemia, particularly when granulomatous disease, lymphoma, or CYP24A1 deficiency is suspected
As a second-line test following measurement of 25-hydroxyvitamin D when clinically indicated
CPT Code Information
82652
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DHVD | 1,25-Dihydroxyvitamin D, S | 62290-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8822 | 1,25-Dihydroxyvitamin D, S | 62290-2 |
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.Last updated 29/06/2026