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HelpTest Code AMIT Amikacin, trough
Laboratory Code
LAB5030
Specimen Requirements
Submit only one of the following:
Preferred
Specimen Type: Serum
Container/Tube: Gold Top (SSTII)
Volume: 5.0 mL
Minimum Volume: 0.5 mL serum
Collection Instructions:
Route: Intravenous / Intramuscular
Peak: 30 min – 1 hr after infusion / after 1 hr intramuscular dose
Once daily dosing: Refer to in-house guidelines or pharmacist’s TDM input.
Allow specimen to clot for 30 mins and centrifuge within 2 hrs.
Acceptable
Specimen Type: Plasma
Container/Tube: Green Top (Li Heparin)
Volume: 4.5 mL
Minimum Volume: 0.5mL plasma
Collection Instructions:
Route: Intravenous / Intramuscular
Peak: 30 min – 1 hr after infusion / after 1 hr intramuscular dose
Once daily dosing: Refer to in-house guidelines or pharmacist’s TDM input.
Centrifuge within 2 hrs.
Specimen Stability
| Specimen Type | Serum/Plasma |
|---|---|
| Ambient | Not available |
| Refrigerate | 7 days |
| Frozen | 14 days |
Methodology
Particle-Enhanced Turbidimetric Inhibition Immunoassay (PETINIA)
Days and Times Performed
24 hrs, TAT 1 day
Specimen Condition
| Haemolysis | OK |
|---|---|
| Icterus | OK |
| Lipaemia | OK |
| Others | Not applicable |
Reference Interval
| Traditional dosing | |
|---|---|
| Peak | 25 – 35 mg/L |
| Trough | < 4 – 8 mg/L |
Once daily dosing: Refer to Nomogram or in-house guideline for Amikacin
Critical value: > 35.0 mg/L
Testing Laboratory
National University Hospital
Clinical Chemistry, 67724346
Useful For
Therapeutic drug monitoring.
Service Code
31804
LOINC Code Information
3321-7
Alias
Amikin (Amikacin Sulfate)
Update
Last updated 10/03/2025