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Test Code AMIT Amikacin, Trough

Specimen Requirements

Submit only one of the followings
 

Preferred
Specimen Type: Serum
Container/Tube: Gold Top (SSTII) 
Volume: 5.0mL
Minimum Volume: 0.5mL
Collection Instructions: Collection Time: Route: Intravenous / Intramuscular

Peak: 30 min – 1 hr after infusion / After 1 hour intramuscular dose
Once daily dosing: Refer to in-house guidelines or pharmacist’s TDM input 

 

Allow specimen to clot for 30 minutes and centrifuge within 2 hours.

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Acceptable
Specimen Type: Plasma
Container/Tube: Green Top (Li Heparin)
Volume: 4.5mL

Minimum Volume: 0.5mL plasma
Collection Instructions: Collection Time: Route: Intravenous / Intramuscular

 

Peak: 30 min – 1 hr after infusion / After 1 hour intramuscular dose
Once daily dosing: Refer to in-house guidelines or pharmacist’s TDM input 
 

Centrifuge within 2 hours.

Sample Stability

** Preferred temperatures for storage prior to and during shipping to NUH **

 

Specimen Type: Serum/Plasma
Ambient: Send the sample to the laboratory immediately.
Refrigerate: 7 days
Frozen: 14 days

 

Reference Interval

  4.0-8.0 mg/L
Critical >35.0 mg/L

 

Traditional dosing
Peak 25-35 mg/L 
Trough <4-8 mg/L 

 

Once daily dosing: Refer to Nomogram or in-house guideline for Amikacin

Days and Times Performed

24 hours, TAT 1 day

Methodology

Particle-enhanced turbidimetric inhibition immunoassay (PETINIA)

Service Code

31804

Update

Last updated 26/02/2022

Unacceptable Condition

 

Haemolysis NA
Icterus NA
Lipaemic NA
Others NA

 

Testing Laboratory

National University Hospital

Clinical Chemistry, 67724346

Useful For

Therapeutic drug monitoring