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HelpTest Code 25OHD Vitamin D 25-Hydroxy
Laboratory Code
LAB535
Specimen Requirements
Specimen Type: Serum
Container/Tube: Gold Top (SSTII)
Volume: 5.0 mL
Minimum Volume: 0.5 mL serum
Collection Instructions: Allow specimen to clot for 30 mins and centrifuge within 2 hrs.
Specimen Stability
| Specimen Type | Serum |
|---|---|
| Ambient | 72 hours |
| Refrigerate | 12 days |
| Frozen | 1 year |
Methodology
Chemiluminescent Immunoassay
Days and Times Performed
24 hrs, TAT 1 day
Specimen Condition
| Gross Haemolysis | Alert/Reject |
|---|---|
| Icterus | OK |
| Lipaemia | Alert/Reject |
| Others | Not applicable |
Reference Interval
| Deficient | ≤ 20 ug/L |
|---|---|
| Insufficient | 21 – 29 ug/L |
| Sufficient | 30 – 100 ug/L |
| Possible toxicity | > 100 ug/L |
Testing Laboratory
National University Hospital
Clinical Chemistry, 67724346
Useful For
Diagnosis of vitamin D deficiency and hypervitaminosis D.
Monitoring vitamin D replacement therapy
Service Code
31036
LOINC Code Information
1989-3
Alias
25-OH Vitamin D2 and D3, Serum
Update
Last updated 13/03/2025