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Test Code 25OHD Vitamin D 25-Hydroxy

Additional Codes

Epic Ordering Code LAB535
NBS code LBC00007
Service code 31036

Specimen Requirements

Submit the following:

Container:
Yellow Top

Processing Requirements

Allow specimen to clot for 30 mins and centrifuge within 2 hours

Specimen type: Serum

Volume: 0.5mL

Specimen Stability

Specimen Type Serum
Ambient 72 hours
Refrigerate 12 days
Frozen 1 year

Specimen Condition

Gross Haemolysis Alert/Reject
Icterus OK
Lipaemia Alert/Reject
Others Not applicable

Methodology

Chemiluminescent Immunoassay

Days and Times Performed

24 hrs, TAT 1 day

Reference Interval

Deficient  ≤ 20 ug/L
Insufficient 21 – 29 ug/L
Sufficient 30 – 100 ug/L
Possible toxicity  > 100 ug/L

Testing Laboratory

National University Hospital

Clinical Chemistry, 67724346

Useful For

Diagnosis of vitamin D deficiency and hypervitaminosis D.

Monitoring vitamin D replacement therapy

Update

Last updated 28/07/2026

LOINC Code Information

1989-3

Alias

25-OH Vitamin D2 and D3, Serum